Gruday O., Anisimova O., Moryleva O.,
NATIONAL INSTITUTE FOR HEALTH DEVELOPMENT INNOVATION TECHNOLOGIES,
MOSCOW.
The present theses are part of the scientific work on the study of native blood at various functional and pathological states of organism. The purpose of this research is to study the effect of hydration on the rheological properties of blood by microscopy of the native preparation. Hemorheology defined functional state formed elements and plasma composition (viscosity and osmolality).
In addition, one of the top leading to rheology (fluidity) of the blood is an indicator such as the volume ratio of cell mass to the liquid portion of the plasma. This ratio is, in fact, determined by the amount of water in the vascular channel, and therefore reflects the hydration of the body. The level of hydration is essential for its functioning as a whole, the implementation of adaptation mechanisms of a healthy person and the progress of various pathological conditions. However, to date there are no accepted methods for the assessment of hydration. Practically the only indicator of hydration of the body is the hematocrit. An alternative, very quick and simple method to estimate is to investigate of native blood. The method of the microscopy of native blood (MNB) assumes viewing of a material (blood) right after a capture within 5-10 min. Research is made with video registration and the computer analysis of the received images. At thus correctness of received result is largely determined by the correctness of the preparation making technique.
The study involved 250 people who were divided into 2 groups, comparable in age: Group 1 - no significant pathology identified (67), Group 2 consisted of those with compensated forms of diabetes and coronary heart disease (183 people).
All patients completed the study of native blood twice. Before the first study, all patients within 2 weeks were recommended diet that limits solid food up to 500 ml per day. Clear water practically wasn't used by them. Before the second study examinees in both groups during the same period used up to 1.5 - 2 liters of fluid per day. Their daily ration was included clean drinking water in the amount of up to 1-1.5 liters per day. We evaluated the relation of plasma and formed elements, the quality of the plasma and blood cell morphology. After the first study both groups of patients were identified displacement ratio plasma - forming elements in the predominance of formed elements. Also, all patients had an expressed presence of sludge - a syndrome. The size of red blood cell units consisted of 5-7 or more items.
After the second study, 78% of patients in both groups, the volume ratio of plasma - forming elements were approximately equal to or shifted towards predominance of plasma. Sludge - syndrome or absent (26%) or was expressed slightly (74%). The size of red blood cell units does not exceed the 5 elements.
Based on the foregoing, we conclude that the level of hydration effect on the rheological properties of blood and can be evaluated using the method of native blood microscopy.